Promotores: J.N. Kok, H.J. Hoogeboom

Promotor: D. Bouwmeester, Co-promotor: D. Kraft

Identification and characterization of components of DNA repair pathways and their role in Agrobacterium T-DNA integration and repair of CRISPR/Cas induced DSBs.

Promotor: P. J. J. Hooykaas Co-promotor: B. S de Pater

Promotor: R.D. Gill, F. Taroni

Inactivating mutations in BRCA1 or BRCA2 genes predispose to several types of cancer.

Our DNA molecules are packaged by proteins in compact structures. The aim of this project is to understand how modern gene editing techniques nevertheless get access to their target in the DNA.

The four possible segments A, T, C and G that link together to form DNA molecules, and with their ordering encode genetic information, are not only different in name, but also in their physical and chemical properties.

What is the most effective way to eliminate tumour cells? The DNA repair mechanism could play an important role in increasing the effectiveness of chemotherapy in the fight against cancerous cells. If we are to influence this mechanism, we need fundamental knowledge about how the mechanism works.

The main purpose of DNAmarkerpoint is to better understand the ecology, evolution and biodiversity through the study of ancient- and modern DNA.

Self-assembly is an abundant process in nature and is vital to many processes in living organisms.

Leiden physicist Maarten Kruithof has discovered how our DNA is infallibly able to fold itself and to unfold again. In his dissertation he demonstrates how DNA is folded in a single long, very flexible spiral.

The DNA of young people is regulated to express the right genes at the right time. With the passing of years, the regulation of the DNA gradually gets disrupted, which is an important cause of ageing. A study of over 3,000 people shows that this is not true for everyone: there are people whose DNA appears…

Theoretical physicist Helmut Schiessel receives an NWO Projectruimte grant of 390,000 euro. He will use this budget to hire a postdoc and a PhD student to continue his research on flexibility of double stranded DNA.

Large carnivores and humans, along with their livestock, have co-existed for thousands of years. However, human population growth and an increase in economic activities are modifying the landscape for large carnivores and their prey.

Single-celled organisms have stiffer DNA than multicellular lifeforms like humans and rice. Theoretical physicists managed to simulate the folding in full genomes for the first time to reach this conclusion. Publication in Biophysical Journal on February 7.

Conference

PhD student Willem Noteborn has investigated supramolecular structures. These can be useful for the loading of medicines and signalling molecules regarding, for example, cellular differentiation. In his thesis, he describes the functioning of these structures.

All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).

Why do opposites attract? According to Karin van der Tuin, researcher at Leiden University Medical Center (LUMC), it could be because of your DNA. The more you differ from one another, the better. She will be testing her ideas at the Lowlands festival Lowlands, where she will subject visitors to DNA…

A group of single-celled organisms organises its DNA in a similar way to higher organisms such as plants, animals, and fungi. However, the way packaged DNA is read out differs between the two related groups, Bram Henneman discovered. PhD defence on 5 December.

In an Advance Online Publication biophysicist John van Noort and others show using magnetic tweezers that DNA is folded in compliant helices of chromatin. This allows enzymes access to the DNA needed for gene expression. Van Noort's research group made the discovery in close partnership with researchers…

This project aims to assess the risk of spread of antimicrobial resistance genes in the environment derived from currently used synthetic biology approaches in the field of drug discovery.

Leiden and Jülich researchers discovered an elegant and simple approach to improve the sensitivity of graphene biosensors. These so-called ‘next generation graphene electronic biochemical sensor devices’ are able to detect very low amounts of HIV DNA thanks to their very low electronic noise.

Dynamics of material, social and ideological relationships in the pre-Columbian insular Caribbean

Leiden theoretical physicists have proven that not only the genetic information in DNA determines who we are, but also DNA’s mechanics. Helmut Schiessel and his group simulated many DNA sequences and found a correlation between mechanical cues and the way DNA is folded. Publication in PLoS One.

How do forensic scientists deal with complex DNA-evidence found at crime scenes? Giulia Cereda developed new statistical models to analyse them. PhD defense 12 January.

Researching DNA material for genetic disorders using the most powerful apparatus. This is what GenomeScan, a company on the Leiden Bio Science Park, does. Master's student Konstantina Konstantinopoulou is doing an internship there. 'It's a world where developments happen really rapidly so it's a fantastic…

Promotor: J.N. Kok, P.E. Slagboom Co-promotor: J.F.J. Laros

How is Pol θ responsible for T-DNA integration, and how do other DNA double-strand break repair pathways interact with Pol θ? How may we manipulate T-DNA integration to stimulate error-free integration at a predetermined genomic site?

In human cells, a meter-long DNA is condensed inside a micrometer-sized cell nucleus. Simultaneously, the genetic code must remain accessible for its replication and transcription to functional proteins.

Promotor: A. Kros, Co-promotor: R.R.C.L. Olsthoorn

What is the population size/structure and distribution of tiger and leopard inside the Bardia National Park (BNP) compared with the population outside? What is the movement and home range and conflict pattern in time and space of tigers and leopards inside the BNP compared with outside? What constitutes…

Are traces of fire use detectable in ancient hominin genomes?

How do our cells keep DNA stored away? To find out, biochemist Remus Dame received a 3 million euro Science-Groot grant. Leiden University will share the grant with TU Delft, VU Amsterdam and the Hubrecht Institute.

Leiden physicists and chemists have managed to bring two graphene layers so close together that an electric current spontaneously jumps across. In the future this could enable scientists to study the edges of graphene and use them for sequencing DNA with a precision beyond existing technologies. Publication…

Schneider

The way DNA folds, largely determines which genes are read out. John van Noort and his group have quantified how easily rolled-up DNA parts stack. This costs less energy than previously assumed. Publication in Biophysical Journal.

The way genes are folded determines their activity in our body. Leiden biophysicists are now the first to unravel this structure molecule-by-molecule. Publication on December 1st in Nature’s Scientific Reports.

Promotores: Prof. Dr. Th.J.C. van Berkel, Prof. Dr. M. van Eck

Promotor: E.F. Smets Co-promotor: J. Geml

A collaboration between the IBL and LUMC has resulted in the discovery that the polymerase theta enzyme is essential for the integration of Agrobacterium T-DNA into the genome of plants. The finding means a break-through for the development of more efficient systems for targeted genome modification…

Genes are active sometimes, and other times they remain dormant for a while. Leiden physicist John van Noort receives an NWO ECHO grant to find out how this happens.

More than 60 European researchers and industry partners have set out their roadmap for the application of graphene in marketed products. Leiden chemist Grégory F. Schneider believes that graphene and other layered materials can in the future be used for DNA sequencing applications.

Alireza Mashaghi and his research team have created a new framework to understand shapes of proteins and DNA. With this framework, many diseases can be understood better.