Do large deletions represent a risk during CRISP/CAS9-mediated genome editing in plants?

The molecular mechanisms that instigate a healthy cell to become malignant are fueled by (epi)genetic alterations in so-called driver genes.

Increasing the efficiency of gene targeting (GT) as a genome editing tool in plants has been an important goal in plant biotechnology.

Efficient gene-targeting during meiosis

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

Drug development is a time- and resource-consuming process that starts with the discovery and validation of a (protein) target that contributes to pathogenesis or disease progression.

In this thesis, we used genetically engineered mouse models to identify genes and pathways that are involved in ILC formation and in the development of resistance to FGFR-targeted therapy.

Chemical tools to monitor drug-target engagement of endogenously expressed protein kinases are highly desirable for preclinical target validation in drug discovery. Here, we describe a chemical genetics strategy to selectively study target engagement of endogenous kinases.

Is translocation of nucleases possible via Agrobacterium T4SS and is it efficient enough for mutagenesis?

The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.

John van Noort

Identification and characterization of components of DNA repair pathways and their role in Agrobacterium T-DNA integration and repair of CRISPR/Cas induced DSBs.

Biosynthetic gene clusters (BGCs) for natural products are widespread in microbial genomes, and they are rapidly exchanged. This research assesses the factors that control the spread of BGCs and resistance genes in nature. This includes risk assessment for the spread of engineered DNA in nature.

Aspergillus niger is an important industrial producer of organic acids and enzymes producing large amounts of spent fungal biomass.

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Promotores: T.H.W. Bäck, A. Plaat, Co-promotor: F.J. Verbeek

The Academic Language Centre interpreting and translation service has extensive experience in translating to and from Dutch and English. Our services are characterised by professionalism, quality, and reliability.

The objective of this study was to investigate the expression and function of GRHL2 in different breast cancer subtypes.

How do mycobacteria subvert the defenses of host immune cells?

Promotores: A.H. Meijer, H.P. Spaink

The Summer School in Languages and Linguistics has been a regular event on the Leiden Centre for Linguistics calendar for over 14 years.

This project aims to assess the risk of spread of antimicrobial resistance genes in the environment derived from currently used synthetic biology approaches in the field of drug discovery.

Promotor: P. J. J. Hooykaas Co-promotor: B. S de Pater

Promotor: Prof.dr. G.P. van Wezel

In this special edition of Information Polity there is a focus on the transparency challenges of using algorithms in government in decision-making procedures at the macro-, meso-, and micro-levels.

Streptomyces bacteria are a valuable source of natural products, many of which are used in the clinic or in biotechnology.

Nucleoid associated proteins maintain the architecture of the bacterial chromosome and regulate gene expression, hinting that their role as transcription factors may involve local three-dimensional chromosome re-modelling.

This thesis describes the functional analysis of the Arabidopsis AHL15 gene.

Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.

Promotor: P.J.J. Hooykaas, Co-Promotores: P.B.F. Ouwerkerk, M. Wang

Which genes are involved in woodiness and associated traits such as drought tolerance, flowering time, stem elongation, life span, and plant herbivory, and how do these gene regulatory pathways overlap?

Science, Leiden Academic Centre for Drug Research (LACDR)

Our DNA molecules are packaged by proteins in compact structures. The aim of this project is to understand how modern gene editing techniques nevertheless get access to their target in the DNA.

Just Published: EU Banking and Insurance Insolvency (second edition) Authored and Edited by Gabriel Moss QC, Bob Wessels and Matthias Haentjens Published by Oxford University Press

In this Ph.D. thesis, we characterized two novel upstream regulators of JUB1, HB40, and HB22, in Arabidopsis thaliana. In Chapter 2, we described functions of HB40 in modulating growth and development via the regulation of JUB1 and GA inactivation genes GA2OXs.

Analysis of drug adversity

DNA carries various forms of information. Out of these forms of information the most well-known is classical genetic information.

The mission of the Plant Science research programme is to contribute to the sustainable production of high quality crops, flowers and high-value bio-based products. This is realised by generating fundamental knowledge of basic biological processes related to development of plants and their interaction…

My PhD project studies how the temporally sequential Hox gene expression is regulated during head-tail patterning of the frog embryo.

Leiden biologists have shown that a gene present in plants, animals and yeasts does play an important role in plants, although for years the gene was considered unimportant. It turns out the gene plays a crucial role in the development of vascular tissue in plants. Publication in Nature Plants on 11…

Plant architecture has distinct forms in different plant species, but also within a species the finalarchitecture of a plant is determined by its gradual development and changes therein induced by environmental conditions during the plant’s life cycle.

On 12 March 2019, KPN published the sixth edition of the European Cyber Security Perspectives. Sergei Boeke, researcher at the Institute of Security and Global Affairs, has written an article about Cyber ​​Warfare.

The Law of the European Union is a complete reference work on all aspects of the law of the European Union, including the institutional framework, the Internal Market, Economic and Monetary Union and external policy and action.

Introduction to Medieval Europe 300–1500 provides a comprehensive survey of this complex and varied formative period of European history, covering themes as diverse as barbarian migrations, the impact of Christianisation, the formation of nations and states, the emergence of an expansionist commercial…

This project will lead to a critical edition and annotated translation of the Niśvāsamukha, the opening book of the Niśvāsatattvasaṃhitā.

This project is funded by the Dutch Cancer Society. The project will be part of an ongoing research line aimed at identifying mechanisms of cancer therapy resistance and metastasis. In this project, it will be investigated how the Grainyhead family of transcription factors controls therapy response…