Central in coordination and organometallic chemistry is the synthesis of new chelating ligands, the synthesis and characterization of metal complexes with these ligands, and the study of their properties. An important goal in my research is to create understanding of the relation between the structures…

Promotor: Prof.dr. A.P. IJzerman, Co-Promotor: Dr. L.H. Heitman

Supervisor: Anna Vlachodimou

Research question

Supervisor: Gerard van Westen

Before the large scale use of renewable energy sources can be implemented in our society, the storage of electrical energy needs to be tackled. Storage the energy as hydrogen via the reduction of protons is a good option.

PhD Defence

Lecture

In this dissertation, the synthesis and characterization of a series of iron complexes based on different ligand platforms are described.

Promotor: E. Bouwman, Co-Promotor: S. Bonnet

The objective is to develop in vitro antigen degradation assays in order to mimic the fate of the antigen in vivo.

Supervisor: Gerard van Westen

A full overview of MCBIM PhD theses.

A tantalizing concept that has emerged in our recent research is binding kinetics. An important parameter is residence time (RT), a direct reflection of how long a drug stays bound to its target. This parameter is of crucial importance, because drug action lasts only as long as the receptor-ligand complex…

Metal-containing molecules combine geometrical features and a reactivity that are inherently different from that of organic molecules. My research focuses on light-activatable metal-based anticancer drugs and metal-functionalized liposomes. Light is a very selective way to activate photosensitive drugs…

The main research theme in the group of Dennis Hetterscheid is to understand and mimic bioinorganic multi-electron processes that are relevant to our future energy infrastructure. Reduction of protons generates hydrogen that can be used as a chemical fuel. Alternatively to gaseous hydrogen, the reduction…

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

Chemical tools to monitor drug-target engagement of endogenously expressed protein kinases are highly desirable for preclinical target validation in drug discovery. Here, we describe a chemical genetics strategy to selectively study target engagement of endogenous kinases.

Vitamin A or retinol is essential in embryonic development, the visual cycle and the immune system.

This thesis describes the design, characterization and application of dextran based crosslinked network.

.

Coordination chemistry is the chemistry of metal atoms

Can light-activatable ruthenium compounds be used as a more selective anticancer treatment?

Research question

Research at the MCBIM group is comprised of the following research themes:

An integrated approach for the simultaneous prediction of polypharmacology and binding affinity/potency of small molecules.

The Chemical Probe Facility is part of the Leiden Early Drug Discovery & Development (LED3) center. Activity-based protein profiling (ABPP) is one of the pillars of chemical biology. ABPP determines the activity of entire protein families in living cells and tissues under physiological conditions, such…

Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…

Due to their central physiological roles in living organisms, retaining beta-glycosidases have been the subject of tremendous research efforts to examine their structure/function relation using numerous biophysical and biochemical approaches.

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

Mouse models are commonly used to assess the immunogenicity of therapeutic proteins and to investigate the immunological processes leading to antidrug antibodies. The aim of this work was to develop a transgenic (TG) Balb/c mouse model for evaluating the immunogenicity of recombinant human insulin (insulin)…

This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.

PhD Defence

PhD Defence

PhD Defence

Activity-based protein profiling (ABPP, also termed chemical proteomics), is one of the pillars of chemical biology, and at LED3 we have taken it to the next level. ABPP allows the assessment of protein function in live cells and tissues, which means that the activity of a complete protein family can…

Animal models suggest that the chemokine ligand 2/CC-chemokine receptor 2 (CCL2/CCR2) axis plays an important role in the development of inflammatory diseases. However, CCR2 antagonists have failed in clinical trials because of a lack of efficacy.

Promotores: Prof.dr. W.J.G.M. Peijnenburg & Dr. M.G. Vijver

The potential of liposomal cancer vaccines

Tools and Data of the department of Environmental Biology

PNEC-pro is a user-friendly screening tool for professionals dealing with the assessment of surface water quality. The tool calculates local, watertype specific no-effect concentrations (PNEC) of copper, lead, nickel, and zinc based on biotic ligand models (BLMs).

Promotor: A.P. IJzerman Co-promotor: L.H. Heitman

Multicopper oxidase, laccase, can efficiently reduce oxygen to water and are mostly used in the enzymatic biofuel cells.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Despite the fact that gold nanoparticles (GNPs) are one of the most studied nanoparticles, there is still a necessity for new approaches allowing for effective protective coating to enable wider use of GNPs in biomedical applications.

Promotor: M. Ubbink, Co-promotor: G. Siegal

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

The division of Drug Discovery and Safety combines the former divisions Toxicology and Medicinal Chemistry.