Metabolic diseases are a burden on the European population and health care system. It is increasingly recognised that individual differences with respect to history, lifestyle, and genetic make-up affect disease progression and treatment response. A Systems Medicine approach, based on computational…

Promotores: T. Hankemeier; R. Berger, Co-promotor: R.J. Vreeken

We are developing biology-driven platforms to measure metabolites based on liquid chromatography-mass spectrometry (LC-MS), gas chromatography-MS (GC-MS), and capillary electrophoresis-MS (CE-MS).

The challenge of achieving fast quantification in metabolomics is the presence of severe matrix effects during the MS analysis of complex samples.

In this thesis, a system pharmacology approach, integrating metabolomics, pharmacology and chemical biology, was applied to understand and modulate the endocannabinoid system across different model systems (cells, zebrafish, mice and humans).

Can we develop a generic method for Quan/Qual analysis of small molecule drugs, their metabolites and endogenous metabolites in (pre-)clinical samples?

A major goal of ABS research is to overcome one of the major bottle-necks in metabolomic research: the lack of a high-throughput infrastructure in which thousands of samples can be processed in a standardized and cost-effective way (max. a few tens of Euro’s per sample for a full metabolome profile).…

The skin is our natural barrier and lipids are a key part of this barrier. In the outer skin layer, the stratum corneum (SC), lipids form a densely organized structure dependent on the composition of these lipids.

PhD at the Max Planck Institute of Molecular Plant Physiology, Golm – Germany

In order to gain a better insight into the basis of the rapid jasmonic acid (JA)-mediated stress response, an integrated approach using a targeted high-resolution mass spectrometry-based (HRMS) platform, was conducted to monitor the metabolism of JA using the model system of cell suspension cultures…

Can animal venoms yield novel antimicrobial peptides?

The high prevalence of pure red cell aplasia in Thailand has been associated with the sharp increase in number of recombinant human erythropoietin (rhEPO) copy products, based on a classical generic regulatory pathway, which have entered the market.

What are the functions of the endocannabinoid system?

Metabolomicshas emerged as an important discipline to study molecular and cellular processes in living cells and organisms with the ultimate aim to obtain an answer to a given biological/clinical question.

Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…

Promotor: C.A.M.J.J. van den Hondel, Co-promotor: Sandra de Weert

The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.

TRANSVAC is a collaborative infrastructure project funded by the European Commission (EC), initially under the 7th Framework Programme (FP7) and currently under Horizon 2020. The project is a joint effort of leading European groups working in the field of vaccine development, and is coordinated by…

PhD at the Natural Products Laboratory, Institute of Biology, Leiden University - the Netherlands

Can a new proteomics workflow be developed to link genes and gene clusters to bioactive molecules, identify novel compounds and enhance the production in the Streptomyces lividans enzyme?

The Basics and Applications to Plant Sciences will be held physical. See below for more information.

- Which metabolites could be components of NADES? - How can we prove the presence of NADES in nature? - What are the roles of NADES in nature? - How to apply NADES in life sciences?

Science, Institute of Biology Leiden (IBL)

- How to deconvolute metabolic mixture of precursors and products by biontransformation? - How to optimize the reaction conditions to produce bioactive compounds in biotransformation? - What is the effect of co-treating fungi or bacteria for biotransformations?

PhD Defence