The aim of this thesis is to explore the use of Bioorthogonal Antigens to study the cross-presentation pathway.

This thesis describes the use of bioorthogonal proteins in immunological settings. It provides an introduction towards the field of protein modification, which was used throughout the thesis for the expression of proteins containing unnatural amino acids.

The close interaction between organic chemi stry and biology goes back to the late 18th century, when the modern natural sciences began to take shape. After synthetic organic chemistry arose as a discipline, organic chemists almost immediately began to pursue the synthesis of naturally occurring compounds,…

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

Three at the same time: a ligation strategy combining tetrazine–norbornene cycloaddition, Staudinger–Bertozzi ligation, and copper(I)-catalyzed click reaction was used to label the three catalytic activities of the proteasome simultaneously in a single experiment. The orthogonality of the three ligation…

Cytotoxic T-cells (CTLs) are involved in the clearance of viruses and killing of tumor cells.

PhD Defence

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system.

PhD Defence

The Leiden Institute of Chemistry (LIC) is the basis for research and collaborations of the Leiden chemistry groups. Chemistry is the central science enabling a healthy future in a sustainable society. Chemistry researchers at Leiden University take a fundamental approach in finding tailored solutions…

Phosphorylation affects all four major biomolecules – proteins, lipids, carbohydrates and nucleic acids – and plays a pivotal role in the most fundamental cellular functions.

Lecture

PhD Defence

Conference

The goal of target discovery is to identify and validate proteins and biological pathways that are involved in the disease process. Modulating these target proteins and pathways with small molecules, therapeutic proteins or other biomolecules (e.g. mRNA) could deliver an effective and safe drug or v…

Lecture

Promotores: Prof.dr. H.S. Overkleeft, Prof.dr. G.A. van der Marel

Promotor: H.S. Overkleeft, Co-promotor: B.I. Florea

PhD Defence

PhD Defence

PhD Defence

A group of chemists, that includes Sander van Kasteren and Hermen Overkleeft, has discovered that azides, a certain type of chemical compound, can determine whether or not T cells respond to or ignore a vaccine. Their discovery is set to be published in Angewandte Chemie.

Research at the BIOSYN group is comprised of the following research themes:

Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…

The research in the Biosyn group is focused on the design, synthesis and function of the four major types of biomolecules: nucleic acids, carbohydrates, peptides and lipids and hybrid structures thereof. These biomolecules and their derivatives are used in drug discovery and chemical biology, to develop…

On Friday May 3rd 2013 NWO has announced that Dr. Mario van der Stelt, Division of Bio-Organic Synthesis (LIC) and Dr. Laura Heitman, Division of Medicinal Chemistry (LACDR), have been awarded with an ECHO-STIP grant for their project, entitled: “Novel target engagement biomarkers for better drug ca…

Lecture

Lecture

Overview of Molecular Physiology publications