We aim to understand ion homeostasis in the model eukaryote S. cerevisiae.

The Protein Centre can supply you with purified proteins that are essential for many experiments, ranging from in vitro inhibitor screening and enzymatic analysis to manipulation of life cells.

Promotor: Prof.dr. E.J.J. Groenen

How can we develop a new microscope that can distinguish individual atoms in proteins?

Promotor: Prof.dr. C.A.M.J.J. van den Hondel, Dr. ir. V. Meyer, Co-promotor: Dr. A.F.J. Ram

Oxidation is a common degradation pathway that affects therapeutic proteins and peptides during production, purification, formulation, transportation, storage and handling of solid and liquid preparations. In the present work we review the scientific literature about structural and biological consequences…

Promotor: M. Ubbink, Co-promotor: G. Siegal

Promotor: M. Ubbink, Co-promotor: G. Siegal

Supervisor: P.J.J. Hooykaas Co-Supervisor: R. Offringa

Promotor: W. Jiskoot, Co-promotor: A. Hawe

Promotor: H.S. Overkleeft, Co-promotor: M. van der Stelt

Promotor: M. Ubbink, Co-promotor: G. Siegal

In our research protein translocation from Agrobacterium into yeast and plant cells is studied to obtain fundamental insights in the translocation process

In the last decades, activity-based protein profiling (ABPP) has emerged as a powerful chemical tool that may aid the ever-challenging drug discovery process.

Glycosidases are important enzymes in the turnover of polysaccharides and glycoconjugates, and are involved in a range of human pathologies including genetic disorders such as Gaucher and Pompe disease, but also in various cancers.

In this study, we evaluated the influence of supercritical carbon dioxide (scCO2) spray drying conditions, in the absence of organic solvent, on the ability to produce dry protein/trehalose formulations at 1:10 and 1:4 (w/w) ratios.

Hass

The RNA guided endonuclease based on the CRISPR/Cas system of Streptococcus pyogenus is a potent new tool for genome engineering in plants.

Promotor: W. Jiskoot, Co-promotor: H.A. Every

The mission of the Plant Science research programme is to contribute to the sustainable production of high quality crops, flowers and high-value bio-based products. This is realised by generating fundamental knowledge of basic biological processes related to development of plants and their interaction…

Therapeutic proteins have revolutionized the treatment of many severe diseases like multiple sclerosis, haemophilia, diabetes and many more. Unfortunately, even recombinant proteins with structures (almost) identical to those of their endogenous counterparts often induce the formation of anti drug antibodies…

The drug BIA 10-2474 inhibits fatty acid amide hydrolase (FAAH), a lipase that degrades a specific endocannabinoid. On the basis of this activity, BIA 10-2474 was being developed as a potential treatment for anxiety and pain. In a phase 1 trial of the drug, one subject died, and four others suffered…

Promotor: Prof.dr. M. Ubbink

Is translocation of nucleases possible via Agrobacterium T4SS and is it efficient enough for mutagenesis?

This thesis describes the importance of being able to control the selectivity of potential drug candidates.

The main subject of this thesis is the design and synthesis of paramagnetic molecules for protein studies with NMR and EPR spectroscopy.

The aim of the research presented in this thesis was to develop new methods forchallenging systems in liquid-state NMR using paramagnetic effects generated by thetwo-armed probe CLaNP-5.

Bacteria often experience external challenges, such as changes in environmental conditions or attacks by bacteriophages.

Determining new protein structures from X-ray diffraction data at low resolution or with a weak anomalous signal is a difficult and often an impossible task.

Promotor: Prof.dr. M. Ubbink

Promotor: Prof.dr. J. Brouwer, Co-promotor: Dr. Remus Th. Dame

Promotor: A. Kros

The research is aimed at development of an efficient gene targeting method that allows controlled integration of DNA at a preselected site in the Trichoderma genome.

Supervisor: P.J.J. Hooykaas

The thesis describes the use of electron paramagnetic resonance (EPR) spectroscopy, in continuous wave and pulse modes, to address the interaction of α-Synuclein (αS) with membranes and the aggregation of αS.

The close interaction between organic chemi stry and biology goes back to the late 18th century, when the modern natural sciences began to take shape. After synthetic organic chemistry arose as a discipline, organic chemists almost immediately began to pursue the synthesis of naturally occurring compounds,…

Promotores: H.S. Overkleeft, G.A. van der Marel, Co-promotor: B.I. Florea

The present thesis focuses on the pharmacological concept of drug-target interaction, which dates back to the beginning of modern pharmacology.

Source: Purinergic Signal., Volume 10, Issue 3, pp. 441-53 (2014)

SMR can be used to measure the mass of adsorbed protein to nanoparticles with a high precision in the presence of free protein.

Promotor: A.P. IJzerman

The barrier function of the skin is primarily provided by the stratum corneum (SC), the outermost layer of the skin. Skin barrier impairment is thought to be a primary factor in the pathogenesis of atopic eczema (AE).

Can we find new chemical and biological sensing routes on the edge and surface of graphene to improve the potential of graphene to act as a sensor?

The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.

It is estimated that approximately 30% of all proteins require a metal to function. Investigating the relationship between metal-binding and peptide/protein folding allows us to uncover fundamental rules for creating metallo-peptides and proteins, which in turn leads to the creation of new structures,…

PNAS publication on protein encounter complexes

Science, Leiden Institute of Chemistry (LIC)

This thesis describes the structural and biochemical characterization of the β-lactamase BlaC from Mycobacterium tuberculosis (Mtb), and the Alr and YlmE proteins from Streptomyces coelicolor A3(2).Mtb is the main cause of tuberculosis.

Promotor: M. Ubbink, Co-Promotor: R. T. Dame