Universiteit Leiden

nl en

Publication

Automatic protein structure solution from weak X-ray data

Determining new protein structures from X-ray diffraction data at low resolution or with a weak anomalous signal is a difficult and often an impossible task.

Author
Skubak, P., N.S. Pannu
Date
15 November 2013
Links
DOI link

Here we propose a multivariate algorithm that simultaneously combines the structure determination steps. In tests on over 140 real data sets from the protein data bank, we show that this combined approach can automatically build models where current algorithms fail, including an anisotropically diffracting 3.88 Å RNA polymerase II data set. The method seamlessly automates the process, is ideal for non-specialists and provides a mathematical framework for successfully combining various sources of information in image processing.

This website uses cookies.  More information.