Universiteit Leiden

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Lecture

Correlative Light Electron Microscopy: 1 + 1 = 3

  • Dr. Paul Verkade (Wolfson Bioimaging Facility, University of Bristol)
Date
Tuesday 1 November 2016
Time
Location
Gorlaeus Building
Einsteinweg 55
2333 CC Leiden
Room
GM4.13

Abstract

Correlative Light Electron Microscopy (CLEM) combines the strengths of light and electron microscopy in one experiment and the sum of such an experiment should provide more data / insight than each technique alone (1 + 1 = 3). There are many ways to perform a CLEM experiment but the experimental set up should primarily depend on the biological question to be answered.

Sometimes a fairly simple approach suffices to answer the question. In other cases however it requires the development of new tools and instruments to adequately do so. In my presentation I will highlight this approach through a number of examples.

References

  • Olmos, Y. L. Hodgson, J. Mantell, P. Verkade, and J.G. Carlton (2015). ESCRT-III controls nuclear envelope reformation. Nature, 522: 236–239.
  • Hodgson L, D. Nam, J. Mantell, A. Achim, and P. Verkade. (2014). Retracing in Correlative Light Electron Microscopy: Where is My Object of Interest? Methods in Cell Biology, Volume 124: Correlative Light and Electron Microscopy, 1-21.
  • Hodgson L, J. Tavaré, and P. Verkade. (2014). Development of a quantitative Correlative Light Electron Microscopy technique to study GLUT4 trafficking. Protoplasma. 251:403-416.
  • Brown, E., J., Van Weering, T. Sharp, J. Mantell, and P. Verkade (2012). Capturing endocytic segregation events with HPF-CLEM. Methods in Cell Biology, Volume 111: Correlative Light and Electron Microscopy, 175-201.

This seminar will be at 15h30 in room GM4.13 (the new building) located on the 4th floor between the West and East G-wing. Afterwards there will be time for discussion, drinks and snacks.

Wolfson Bioimaging Facility, University of Bristol

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