Promotie
Transplantation of cultured corneal endothelial cells: towards clinical application
- D. Spinozzi
- Datum
- dinsdag 17 november 2020
- Tijd
- Locatie
-
Academiegebouw
Rapenburg 73
2311 GJ Leiden
Samenvatting
Corneal transplantation still represents the elected method for the treatment of corneal endothelial pathologies. However, the worldwide shortage of donor corneas induced the exploration of approaches to use the donor tissue more efficiently or to be more independent from donor tissue.
This thesis will illustrate the improvements of new strategies for cell-based corneal endothelial regeneration, alternative to corneal endothelial surgical transplantation, by bridging the gap between in vitro experiments and clinical models. In the studies described, we first address the establishment of a GMP-compliant protocol for in vitro human corneal endothelial cell (hCEC) culture for clinical application and then we focus on endothelial cell sheet transplantation, describing both in vitro and in vivo applications of expanded CEC-carriers constructs made by biocompatible materials.
Promotor
- Prof.dr. M.J. Jager
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