Promotie
Modeling Vascular Diseases Using Human Induced Pluripotent Stem Cells
- X. Cao
- Datum
- woensdag 9 september 2020
- Tijd
- Locatie
-
Academiegebouw
Rapenburg 73
2311 GJ Leiden
Samenvatting
In depth analysis of endothelial cells (ECs) and myeloid cells differentiated from human induced pluripotent stem cells (hiPSCs) were shown in this thesis to be very similar to tissue derived equivalents in vivo. They thus provided an essential tool to study their biology and various vascular and inflammatory diseases that are caused by their dysfunction.
In this thesis, we first established an efficient protocol to derive fully functional monocytes and different subtypes of macrophages from hiPSCs in order to provide an inflammatory component for modelling of vascular diseases in vitro. Next, we characterized the developmental path of hiPSC-derived ECs (hiPSC-ECs) using an ETV2mCherry reporter hiPSC line which we had generated earlier using Crispr-Cas9 gene targeting. These hiPSC-derived cells were then used to model two vascular diseases Hereditary Hemorrhagic Telangiectasia type 1 (HHT1) and Pseudomyogenic hemangioendothelioma (PHE) in vitro. These models provided valuable tools to investigate underlying pathologies of human vascular diseases and contributed to moving this area of research forward.
Promotor
- Prof.dr. C.L. Mummery
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