Lezing
Joan van der Waals colloquium
- Datum
- vrijdag 10 mei 2019
- Tijd
- Toelichting
- Drinks afterwards.
- Locatie
-
Oort
Niels Bohrweg 2
2333 CA Leiden - Zaal
- De Sitterzaal
How fast are functional motions of protein machines? A single-molecule perspective
Protein machines carry out multiple tasks in the cell, but their modes of operation are still quite obscure. Single-molecule fluorescence spectroscopy is a powerful tool for exposing function-related motions of such nanomachines. We recently developed novel methodology that allows us to track protein dynamics even on the microsecond time scale. In the lecture, I will show how this analysis helped us understanding the dynamics of two different machines. Surprisingly, internal motions are found to be faster than chemical steps by 2-3 orders of magnitude, which we will try to rationalize in the lecture.